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After incubation, the cells were washed with phosphate-buffered saline (PBS) and processed for posterior studies as follows: –Immunofluorescence studies (IF): Cells were fixed with 4% paraformaldehyde, incubated with Hoechst (5 µg/ml) for 30 m, washed with PBS, and mounted using Vectashield mounting media (Vector Laboratories, CA, USA)