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On the other hand, the production of 8-OHdG is not associated with the remaining clinical parameters, such as age, GFR, HCT, and BSA
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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm
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