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brocco-glutathione black stool Exogenous myrosinase from mustard seed increases bioavailability of sulforaphane from a glucoraphanin-rich broccoli seed extract in a randomized clinical study diet coffee
AOD 9604 - 5MG
Nutritional Support Wound healing demands increased nutritional resources
diet coffee
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coli cells expressing GST fusion protein in pGEX vector Isopropyl-1-thio-β-D-galactopyranoside (IPTG), 100 mM stock 2.3 Affinity purification of GST fusion protein Glutathione Sepharose 4B bulk matrix (GE Healthcare or Sigma) (see Note 3) Column (BioRad 2.5 cm × 10 cm Econo-Column) 10 mM glutathione buffer: 50 mM Tris, 10 mM reduced glutathione, pH 8.0 (make fresh daily) PBS/EDTA/PMSF: 1X PBS, 5 mM EDTA, 0.15 mM PMSF, pH 7.4 Pelleted E
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