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Since the Injection procedure does not include the components of a Preventive Medicine E/M service, the Injection can be reported separately and the Preventive Medicine E/M code does not need a modifier to indicate it is distinct or separate from the Injection procedure

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In brief, bone marrow cells were cultured at a concentration of 3×10 5 cells/ml in RPMI-1640 medium containing 10% FBS supplemented with 200 ng/ml recombinant murine Flt3L (rmFlt3L, PeproTech, Rocky Hill, NJ, USA) and 2-mercaptoethanol (50 μM, Sigma-Aldrich), for 8 d