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Briefly, 100 μl of enzyme conjugate was added to the wells containing 50 μl of standard or plasma, covered with an adhesive strip and incubated for 60 min at 37 °C

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Photosensitivity and skin fragility found in patients with cutaneous porphyrias, VP and HCP, occur independently of acute attacks and do not occur in AIP.4,5 When acute porphyria is suspected, biochemical plasma or urinalyses of porphyrin precursors are mandatory to confirm the diagnosis of an acute porphyric attack.2 More than five-fold elevation of urinary PBG excretion together with typical symptoms of an acute attack is sufficient to start a treatment,6 but in each case other causes of abdominal crisis must be excluded before a specific treatment of an acute attack is administered

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