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Western blotting For western blot analysis, cell lysis was performed using a lysis buffer composed of 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5% NP-40, 0.5% Triton X-100, 0.1% Na-deoxycholate, and 1 mM EDTA supplemented with a protease inhibitor cocktail
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Specification:20 mg
10.1093/nar/20.6.1425 Nucleic Acids Res
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