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This decreased expression inhibited dendritic cell maturation and impaired the initiation of immune responses.14,15,61 Ethanol consumption also shifted the cytokine profiles of dendritic cells, often reducing proinflammatory IL-12 while increasing anti-inflammatory IL-10, thus promoting immune tolerance over activation and increasing infection susceptibility.13 Moreover, ethanol-fed mice exhibited a decreased total number of dendritic cells in the lungs following influenza virus infection.62 Pulmonary dendritic cells from ethanol-treated mice (20% EtOH in the water for 4 to 16 weeks) also failed to upregulate CD40 and CD80 expression in response to stimulation of toll-like receptors on their surface by antigen binding.63 Lack of CD40 and CD80 expression significantly impaired the dendritic cells’ ability to stimulate T-cell activation and differentiation.64 Thus, ethanol impaired the ability of dendritic cells to induce a robust adaptive immune response to infectious challenge

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