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Given this finding we began examining Nle1- AngIV and found that as C-terminal amino acids were removed Ki decreased from 3.59 × 10-12 for Nle1-AngIV to 4.89 × 10-9 M for Nle1- tripeptide (Nle-Tyr-Ile)

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Therefore, direct target-engagement interpretation should prioritize measurements that sit close to the enzyme reaction itself: NNMT activity 1-MNA formation Nicotinamide availability NAD + -related metabolites SAM SAH The SAM:SAH ratio [9] 5-Amino-1MQ and related methylquinolinium compounds have been investigated as NNMT inhibition tools in laboratory models

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