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Barcode-based demultiplexing was performed using IDEMP software with default parameters ( Obtained reads were assembled, quality controlled, and clustered using Usearch8.1 software package ( Briefly, reads were merged using -fastq_mergepairs –with fastq_maxdiffs 30 and quality filtering was done with fastq_filter (-fastq_maxee 1), minimum read length 200 bp

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Borreliacidal assay Bb , strain B31, was seeded at 1 × 10 5 spirochetes/mL in 96-well round-bottom plates with a 1:10 dilution of heat-inactivated mouse sera, 1:10 guinea pig complement sera (Sigma S1639), 100 μg/mL of LA-2 antibody (Absolute Antibody, Ab01070-3.0) (positive control), and diluted in BSK-H complete media for a total of 100 μL

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