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The first reported derivatization reagent for MALDI-MS thiol detection is (E)-2-cyano-N-(2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)-3-(4-hydroxyphenyl)acrylamide (CHC-Mal), which enables in situ derivatization for spatial distribution analysis of cysteine and glutathione [29]
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Some time after induction exposure, test and control animals are challenged at a distant site with a sub-irritant concentration of the chemical, which is generally lower than the concentration used for induction
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