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Using a standard cur- ve, obtained by precipitating known sulphate concentra- tions, we converted the absorption to mM intracellular sulphate and calculated the rate constant in min-1 by a non-linear, least square, curve-fitting procedure applying the following equation: C (t) = C ∞ (1 – e –rt ) + C 0 where C 0 , C t and C represent the intracellular sulphate concentrations measured at times 0, t and [14,15], e indicates nepero number ( 2.7182818) and r is a constant
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