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glutathione resynthesis Biochemistry | Glutathione Synthesis Enzymes Event Group 10
Riboflavin drives nucleotide biosynthesis and iron-sulfur metabolism to promote acute myeloid leukemia | bioRxiv
doi:10.1186/s12943-024-02217-2 24
Event Group 10
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The retained protein fraction was immediately used for the determination of γ-glutamylcysteine concentration or assayed for GCL activity in 0.1 M Tris–HCl buffer containing 0.15 mM KCl, 20 mM MgCl 2 , 2 mM EDTA, 10 mM ATP, 10 mM L-cysteine, 40 mM L-glutamate and 220 μM acivicin, pH 8.2, at 37 °C for 15 min
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