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Gorter, J

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Size:5gm

Gene transcripts were defined as differentially abundant when the log 2 of fold-change values (log 2 fc) between cultures grown with chlorinated methanes and methanol were ≥2.0 or ≤ −2 (Yang et al., RT-qPCR Retrotranscription was performed from 2.0 μg of DNA-depleted RNA extracted using the Nucleospin RNA plus kit (Macherey Nagel) combined with Turbo DNase and Turbo DNA free kit (Invitrogen) using Superscript III (Invitrogen) and random hexanucleotide primers (Invitrogen) following manufacturers' protocols, except that RNaseOUT™ was replaced by RNasin™ (40 U⋅μL −1