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Annexin V plus membrane-impermeant dye apoptosis staining Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis

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In part, the bimodal effects of NO may relate to differences between its multiple mammalian synthetic enzymes, with neuronal nNOS and vascular eNOS tending to be more neuroprotective, while inflammation-associated glial and immune iNOS activity tends to be more neurotoxic (Puzzo et al., 2006