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36 SUMMARY Spine trauma can lead to a measurable damage up to a complete destruction of the intervertebral disc
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Inhibition of 5-LOX in a Cell-Free Assay The enzyme activity of human recombinant 5-LOX was determined spectrophotometrically by monitoring the oxidation of H 2 DCFDA to the highly fluorescent 2’,7’-dichlorofluorescein during the catalytic reaction mediated by 5-LOX as reported (Wu et al., 2012)
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