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miRNA-seq library construction used a v4 NEXTflex Small RNA-seq kit (PerkinElmer), then samples were barcoded with individual tags following the manufacturer’s instructions

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Additionally, LS-102 stabilizes mitochondrial membrane potential, reduces the Bax/Bcl-2 ratio, and suppresses Caspase-3 expression, thereby alleviating mitochondrial fission-mediated apoptosis ( In conclusion, AS-IV mitigates cardiac I/R injury through multiple molecular mechanisms, including anti-apoptotic, anti-inflammatory, antioxidant, and mitochondrial protective effects