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Characterization of AUA decoding with modified mRNAs To examine the effect of the additional hydrogen bonding mediated by the ava 2 C side chain on AUA decoding, we performed an A-site tRNA-binding experiment using a series of synthetic mRNAs bearing 2′-OH (control), 2′-H, 2′-OMe or 2′-F at the residue 3′-adjacent to the A-site codon (Fig

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In vitro, pharmacologic lessening of mGSH sensitizes hepatocytes to tumor necrosis factor (TNF)-mediated cell death, which parallels the findings observed after alcohol intoxication [75]