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People with copper sensitivity, Wilson disease, liver disease, pregnancy, or complex chronic conditions should avoid GHK-Cu or consult a doctor first, as safety data for these populations remains insufficient
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In brief, cells were washed once with PBS and lysed by the addition of luciferase lysis buffer (1% Triton X-100, 25 mM Glycyl-Glycin (pH 7.8), 15 mM MgSO 4 , 4 mM EGTA, 1 mM DTT)
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