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further notes that B12 injectables should be made available over the counter at pharmacies, which would bring our approach in line with that of other countries, affording those with B12 deficiency the same dignity and control over their own health as a diabetic using insulin, and reducing the workload and financial burden on GP practices, District Nurses and other NHS services

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Two hundred microliters of plasma were extracted with four hundred microliters of −20°C precooled methanol and incubated in −20°C for 45 minutes followed by spinning down in 4°C at 17,000 × g for 30 min to separate supernatant