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Mechanisms: Based on evidence predominantly from cell lines or preclinical models, SCFAs enhance barrier function through different mechanisms: Butyrate provides energy for the colonocytes and regulates hypoxia-inducible factor-1 (HIF-1), which modulates the efficiency of epithelial TJ CLDN1 (gene coding for claudin-1 [60,61]) SCFAs modulate the size and function of the T cell network in the gut through G protein-coupled receptor 43 (GPR43)-dependent mechanisms [62] and through histone deacetylase inhibition [63]
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