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FRAP imaging All live imaging experiments were performed on neurons at DIV 21–23 after transferring infected cultures to a Quick Change Chamber 18 mm Low Profile RC-41LP (Warner Instruments), and keeping them in the original culture media at 37 °C in the constant presence of humidified carbogen for the duration of the imaging
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The amount of NEM remaining after each extraction was evaluated by measuring its concentration in the aqueous phase with HPLC as previously described [17]
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