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The PCR reactions were prepared for three primer pairs in which the internal transcribed spacer 1 (ITS), 5.8S, and ITS2 regions (primers: ITS1 and ITS4), the partial sequence of the gene tef1-α (primers: EF-1αF and EF-1αR), and the partial sequence of the rpb2 gene (primers: fRPB2-5F and fRPB2-7cR) using the EasyTaq ® kit (Sinapse Biotecnologia) using the manufacturer’s recommendations

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Eluted peptides were analysed by the MS in positive polarity using a data-independent acquisition mode as follows: an initial MS1 scan was carried out at 140,000 resolution with an automatic gain control target of 3 × 10 6 for a maximum injection time of 200 ms, m / z range: 410–1,600